《植物生理学报》 2010, 46(2): 147-149

尾叶桉的不同类型愈伤组织芽分化与某些相关生理生化指标的关系

黄真池, 欧阳乐军, 曾富华*, 余秋梅

湛江师范学院生命科学与技术学院, 广东湛江 524048

通信作者：曾富华；E-mail: zengfuhua@gmail.com；Tel: 0759-31 836 22

摘　要：

切取 8 d 苗龄的尾叶桉幼苗下胚轴, 培养 7 d 后愈伤组织的诱导率达 100%。8 周后, 愈伤组织分化成褐色、白色和浅 绿色 3 种类型。其中只有浅绿色愈伤组织能分化出不定芽, 分化率达 57%。浅绿色愈伤组织的过氧化物酶(POD)、超氧化 物歧化酶(SOD)、过氧化氢酶(CAT)和多酚氧化酶(PPO)的活性最高。除 SOD 外, 浅绿色愈伤组织的 POD、CAT 和 PPO 活 性与其他两类愈伤组织相比具有显著性差异(P<0.05)。H2O2 和丙二醛含量则是褐色愈伤组织的最高。

关键词：尾叶桉; 愈伤组织; 过氧化物酶; 过氧化氢酶

收稿：2009-11-27   修定：2009-12-28

资助：广东省自然科学基金(8152404801000011)和广东省科技攻关项目(2006B20101011)。

Relationship between Bud Differentiation and Several Physiological and Biochemical Indexes of Three Types of Eucalyptus urophylla S. T. Blake Callus

HUANG Zhen-Chi, OUYANG Le-Jun, ZENG Fu-Hua*, YU Qiu-Mei

Life Science & Technology School, Zhanjiang Normal University, Zhanjiang, Guangdong 524048, China

Corresponding author: ZENG Fu-Hua; E-mail: zengfuhua@gmail.com; Tel: 0759-31 836 22

Abstract:

Hypocotyls excised from 8 day-old seedlings could dedifferentiate and form callus at 100% when they were incubated on a modified MS (SPCa) medium for 7 days. Fifty-six days later, the callus differentiated into 3 types, including brown callus, white callus and reseda callus. Only could the reseda callus formed adventitious bud at 57% when these calli inoculated with the bud induction medium. The activities of POD, SOD, CAT and PPO of reseda callus were the highest. Compared with the enzyme activity of other types of callus, the POD, CAT and PPO activities of reseda callus showed significantly difference at 0.05 level, except for the activity of SOD. The content of H₂O₂ and malonaldehyde of brown callus was the highest.

Key words: Eucalyptus urophylla; callus; peroxidase; catalase